Laser Force Cytology™ Guides Process Optimization and Bioproduction Outcomes
Process optimization and scale-up considerations serve as linchpins for the successful and efficient production of biopharmaceuticals. Optimization entails fine-tuning various parameters, such as cell culture conditions, media formulations, and purification processes, to enhance productivity and yield while maintaining product quality. Simultaneously, scale-up considerations involve seamlessly transitioning processes from small-scale laboratory settings to large-scale production, ensuring that the increased volume does not compromise the integrity of the final product.
Radiance® enables rapid A/B testing during process development by providing real-time, label-free insight into how cells respond to changes in media formulations, cytokine combinations, donor or cell source selection, transfection conditions, bioreactor parameters, feeding strategies, and other critical process variables. Rather than waiting days or weeks for endpoint assays, developers can assess the cellular impact of process changes as they occur, revealing shifts in vitality, phenotype, stress response, manufacturing readiness, and functional fitness early in development. This accelerated feedback loop allows teams to identify promising conditions faster, eliminate underperforming approaches sooner, and make data-driven decisions with greater confidence. By directly measuring the biological response of the cells themselves, Radiance helps organizations move more efficiently toward a locked process, reducing development timelines and increasing confidence that selected process conditions will support robust manufacturing performance and downstream product quality. The result is faster process optimization, reduced development risk, and a shorter path to clinical advancement.
The real-time, label-free nature of Laser Force Cytology™ enables instant detection of variations or anomalies in cell behavior, allowing for timely interventions and adjustments to maintain optimal process conditions. The integration of Radiance® instrument data further enhances this monitoring by offering comprehensive information on key parameters, facilitating a holistic view of the entire production run. Together, LFC and Radiance® empower biomanufacturers with the tools needed to proactively manage and optimize production processes, ensuring consistency, quality, and efficiency in the manufacturing of biopharmaceuticals.
Real-time Optimization of Culture Conditions
Radiance® Process Development Application Data
Shown in the graph are results from a viral process optimization experiment designed to determine the optimal multiplicity of infection (MOI).
By tracking the Radiance® infection metric in real-time throughout the production process, a variety of process conditions can be quickly screened without having to perform lengthy and resource-intensive assays such as plaque/TCID50, PCR, or ELISA. The infection metric can also be tracked to optimize the harvest timepoint and conditions, allowing for the investigation of a wider range of process conditions than might be possible when analytical results are bottlenecked due to a cumbersome offline assay.
